QX200™ AutoDG™ Droplet Digital™ PCR System

The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR™) applications. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical.

The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time — it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.

Features and Benefits of the QX200 AutoDG Droplet Digital PCR System

Automated Droplet Generator — simplifies the ddPCR workflow and offers flexibility:

·         Generates droplets for 96 ddPCR reactions in under 45 minutes

·         Compatible with both the QX100™ and QX200™ Droplet Digital™ PCR Systems

·         A single AutoDG Instrument can supply 4–5 Droplet Readers continuously

·         Guides setup and loading with large, color touch-screen interface and LED lighting

·         User-definable plate layout with increments of 8 wells provides run-to-run flexibility

·         HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment

Droplet Digital PCR Technology — the most precise and sensitive digital PCR solution for a wide variety of applications, providing:

·         Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays

·         Nanoliter droplet partitioning — reduces bias from amplification efficiency differences and PCR inhibitors

·         Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

·         Cancer biomarker studies and copy number variation — measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. Predesigned, wet-lab validated PrimePCR™ ddPCR™ Assays are now available for mutation and copy number detection.

·         Pathogen detection — employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring

·         Next generation sequencing — perform absolute quantification of an NGS library without the use of a standard curve

·         Gene expression analysis — achieve reliable and reproducible measurements of small fold changes for low-abundance mRNA and miRNA

·         Environmental monitoring — test a wide variety of environmental samples such as soil and water

·         Food testing — perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods

Starting sample size, µl 20
Automated Droplet Generator capacity 1–96 samples/run
Droplets per 20 µl sample 20,000
QX200™ Droplet Reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
Automated Droplet Generator dimensions (W x D x H) Open: 66 x 56 x 89 cm (26 x 22 x 35 in) 
Closed: 66 x 56 x 66 cm (26 x 22 x 26 in)
QX200 Droplet Reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11 in)