QX200™ Droplet Digital™ PCR System

The QX200 Droplet Digital PCR (ddPCR™) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

Benefits of the QX200 Droplet Digital PCR System

·         Most precise and sensitive digital PCR solution for a wide variety of applications

·         Flexible digital PCR chemistry — optimized for TaqMan Hydrolysis Probe and EvaGreen Assays

·         Flexible assay setup — scalable for high sensitivity or high throughput

·         Simple and easy-to-use workflow with 96-sample throughput

·         Droplet partitioning by the QX200 Droplet Digital technology reduces bias from amplification efficiency and PCR inhibitors

·         Convenient assay design — standard curves are not required

Applications of the QX200 Droplet Digital PCR System

·         Cancer biomarker studies and copy number variation 
Measure varying degrees of cancer mutations, detect rare DNA target copies, and resolve copy number variation states with superior sensitivity and resolution. PrimePCR™ ddPCR Assays are now available for mutation and copy number detection. These are predesigned, wet-lab validated assays.

·         Pathogen detection 
Employ the extreme precision of the QX200 System to quantify small fold changes in target DNA or RNA molecules in pathogen detection and monitoring.

·         Next generation sequencing 
Perform accurate quantification and qualification of NGS libraries without the use of a standard curve.

·         Gene expression analysis 
Achieve reliable and reproducible measurements of small fold changes for low abundance of mRNA and miRNA.

·         Environmental monitoring 
Test a wide variety of environmental samples like soil and water using the QX200 System.

·         Food testing 
Perform routine evaluation of genetically modified organisms (GMO) using validated ddPCR methods.

Starting sample size, µl 20
QX200 droplet generator capacity 1–8 samples/cartridge
Droplets per 20 µl sample 20,000
QX200 droplet reader capacity 1–96 samples
Sample illumination Light-emitting diodes
Sample detection Multi-pixel photon counter
Detection channels FAM (EvaGreen), HEX (VIC)
Linear dynamic range 5 orders of magnitude
Precision ±10%
Droplets per 96-well plate, million ~1.5
QX200 droplet generator dimensions (W x D x H) 28 x 36 x 13 cm (11 x 14 x 5")
QX200 droplet reader dimensions (W x D x H) 66 x 52 x 29 cm (26 x 20 x 11")