ZE5™ Cell Analyzer

The ZE5 Cell Analyzer is an innovative new flow cytometer with flexible configurations to meet a broad range of experimental complexities and throughput needs. Whether it’s a low-complexity, or a high-complexity experiment, the ZE5 Analyzer has up to 30 parameters and provides a truly expandable flow cytometer. This flexibility makes the ZE5 Analyzer accessible for novice flow cytometry users yet flexible enough for the most experienced flow cytometry professionals

A fully integrated sample loader with the ability to handle tubes or plates without an instrument hardware change enables true walk-away functionality. Features such as a stat tube station, fluid and reagent hot swap capability, and automated startup and QC allow for continuous operation and maximization of instrument running time for any busy core lab.

The ZE5 Analyzer was created by gathering input from leading flow cytometry experts across the world and was designed to address user-reported gaps in currently available systems. Some of these features include:

Flexible loading options

·         Fully automated sample acquisition from 5 ml sample racks or microplates (up to 384 wells) plus a stat tube option for urgent samples

·         Patent-pending automatic calibration and built-in detection for probe crashes

·         Integrated, programmable wash station reduces carryover and time between runs

·         High-throughput mode can analyze a 96-well plate with < 0.5% carryover in less than 15 minutes

·         Temperature-controlled loading

·         True plate shaker agitation

·         Volumetric sample uptake allows automatic concentration calculation and absolute counting without beads

·         No dead volume requirement, no wasted aspirated sample, and the ability to automatically return unused sample to the tube or well

·         Single-tube load position provides the flexibility to interrupt and load additional samples

·         Add reagents to your samples in real time for kinetic experiments

·         On-board calibration beads provide automated QC without user intervention to reduce setup time

·         Volume tracking for on-board beads provides an alert when replacement is needed

·         Innovative continuous sampling allows unlimited volume acquisition at a stable flow rate

Best-in-class and scalable optics

·         Up to five spatially separated lasers ranging from 355 nm to 640 nm

·         Integrated beam shaping and focusing optics for each laser increases excitation stability

·         Up to 27 fluorescence detectors with FSC/SSC and optional small particle detector (FSC from 405nm laser)

·         Patent-pending ZE5 EYE technology profiles the instrument with 10 LEDs of unique wavelengths

·         ZE5 EYE technology also enables system-to-system detector calibration

·         Dual forward-scatter design allows standard FSC detection while simultaneously collecting small-particle or alternate-wavelength scatter data

·         Field upgradable with modular excitation and detection components

Integrated and easy-to-use fluidics

·         Patent-pending sample delivery system allows stable, continuous flow from 0.0025 to 2.5 µl/s

·         High-velocity sheath flow delivers unparalleled analytical throughput

·         Hot-swappable bulk fluid bottles

·         Built-in ports to connect to a house DI and waste system

·         On-board cleaner and additive bottles

·         Bidirectional flow to the cuvette

·         Sleep mode conserves laser hours and fluids when the system is not in continuous use

Innovative electronics provide the industry’s highest-quality data

·         Measured peak, area, and width parameters available simultaneously for every channel

·         Proprietary digital processing with 24-bit data maximizes dynamic range and reduces channel noise for superior dim-population resolution

·         Zero dead-time processing with dynamic window extension

·         Massively parallel, pipelined architecture eliminates hard aborts

·         Unassisted laser-delay system configures optimal delays

·         Real-time hardware system control

Everest™ Software

Everest Software is the comprehensive instrument control application for the ZE5 Cell Analyzer. Everest Software controls all functions of the ZE5 Cell Analyzer and provides accurate data acquisition and user-friendly data analysis.

Key features of Everest Software include:

·         Automated startup and shutdown

·         Quality control process and onboard QC beads

·         Fluorochrome selector assisting in experimental design

·         Acquisition and file storage for up to 100 million events

·         Multichannel triggering

·         Hit detection

·         Heat map

Fluorescence sensitivity <100 MESF for FITC, PE, and APC
Scatter sensitivity <0.3 μm FSC resolution with small-particle detection module
Sample loading Standard microplates (127.75 x 85.5 mm): up to 384-well
5 ml tubes (12 x 75 mm): stat tube position for single tube, 40-tube rack
Sample throughput <15 min for 96-well plate in high-throughput mode
Excitation sources  Up to five spatially separated lasers. Standard options include:
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
Detection Up to 30 detectors, including FSC and SSC; optional second FSC detector
Cuvette Fused silica with 145 x 265 μm channel
Speed >100,000 events per second with all parameters enabled
Data processing Simultaneous measured peak, area, and width for every channel
24-bit data for peak and area
17-bit data for width with high-resolution linear interpolation at the half height
Fluidics Bulk fluids: 4 x 4 L bulk fluid tanks on board for sheath and waste
Onboard additive concentrate and cleaner
Operational flow rates 0.0025–3.5 μl/sec
Dimensions (W x D x H) 74 x 69 x 66 cm (29 x 27 x 26 in)
Weight 110 kg (240 lb) maximum
Data format Flow Cytometry Standard (FCS) 3.1